Abstract
Colorimetric LOC Compatible with R2R Fabrication For Monitoring the Activity of Enzyme Extracts
Verónica Mora Sanz, Researcher, Tecnalia Research and Innovation
Using a colorimetric Lab-on-a-Chip (LOC) device, the activity of different organic enzyme formulations produced by an efficient fermentation technology are measured. Similar enzyme sensing strategies were chosen to take advantage of synergies in chip design. The methodology consists in a one-step reaction that uses specific substrates to produce a colored end-product. The microfluidic chip design compatible with roll-to-roll (R2R) extrusion coating fabrication consists on an inlet for the enzyme sample, a reaction chamber, and a capillary pump. The enzyme substrates were immobilized on the reaction chamber for simplify the handle for final user. Spotting was employed for the integration of the reagents on the chip because it is a compatible technique with R2R process. COC was chosen for the chip fabrication for its flexibility required in R2R manufacturing and its transparency necessary for colorimetric detection. An adhesive foil is used to cover the structured microfluidic foil. A portable readout device with UV-LED and photodiodes was developed for measuring the enzymatic activity in situ in the manufacturing plant.
Verónica Mora Sanz , Researcher, Tecnalia Research and Innovation
Verónica Mora-Sanz, Ph.D. is a chemist whose current research focuses on optical biosensing methods, development of lab-on-a-chip and the application of nanomaterials into the field of immunosensing. She obtained her degree in Chemistry and her Master degree in Nanostructured materials for Nanotechnological Applications by the University of Zaragoza in 2016. She worked at the Institute of Nanoscience of Aragón in 2016 and 2017 as a novel research. In 2022 she completed her PhD in the University of Basque Country after conducting her research between CIC biomaGUNE and Tecnalia. Her thesis entitled Synthesis and applications in immunoassays of antibody-protected nanoclusters was graduated with honors and comprised two publications and one patent which name is Nanocluster-antibody conjugates and uses thereof. Since 2020 she works as research in the Biomaterials Area of Tecnalia and during these years she has been involved in several R+D projects.
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